Antipruritic properties of topical Channa striatus extract on stratum corneum disruption-itch mice model
Keywords:Antipruritic, Channa striatus extract (CSE), Chronic itch, Tumor necrosis factor-alpha (TNF- α)
Itch is an unpleasant sensation that provokes a strong desire to scratch. In some cases, antihistamines are ineffective in treating chronic itch and produce side effects. Channa striatus fish extract, which can alleviate pain, is also used in this experiment to alleviate the itch. This experiment aims to determine the possible antipruritic effect of topical Channa striatus extract (CSE) on stratum corneum disruption (SCD)-itch mice model. Experiments were divided into 6 groups of male ICR mice which were positive, negative, vehicles, 3% CSE, 7% CSE, and 10% CSE groups. Rostral back of mice was induced with chemicals acetone, diethyl ether (1:1) and water (AcetoneEthylWatermethod) daily for five consecutive days. All groups were treated with specific selective treatments. The scratching behaviour of the mice was observed by videotape. The skin moisture and oiliness scoring was measured daily using a skin analyzer. The skin was isolated for RNA extraction and measured for tumour necrosis factor-alpha (TNF- α) gene expression level using real-time RT PCR. All treatment groups of Channa striatus extract cream had shown a significant reduction in scratching time, increase in skin moisture and skin oiliness. The TNF- α gene expression level had inconsistent results and needed a more extensive sample. The Channa striatus extract cream may be able to alleviate itch compared to the control parameters.
Agner, T. (2016). Skin Barrier Function. Current Problems in Dermatology, 49, 8-26. https://doi.org/10.1159/isbn.978-3-318-05586-3
Dahlan-Daud, C. K., Mat Jais, A. M., Ahmad, Z., Md Akim, A., & Adam, A. (2010). Amino and fatty acid compositions in Haruan traditional extract (HTE). Boletín La-tinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 9, 414–429. http://doi.org/10.1111/j.14401681.2007.04572.x.
Grundmann, S., & Ständer, S. (2012). Chronic pruritus : clinics and treatment. Annals of Dermatology, 1, 1–11. https://doi.org/10.5021/ad.2011.23.1.1
Homey, B., Steinhoﬀ, M., Ruzicka, T., Leung, D. Y. M. (2006). Cytokines and chemokines orchestrate atopic skin inﬂammation. Journal of Allergy and Clinical Immunology, 118, 178–189. http://dx.doi.org/10.1016/j.jaci.2006.03.047
Ikoma, A., Steinhoff, M., Ständer, S., Yosipovitch G., & Schmelz M. (2006) The neurobiology of itch. Nature Reviews Neuroscience, 7, 535–547. https://doi.org/10.1038/nrn1950
James, M., Gibson, R., & Cleland, L. (2000). Dietary polyunsaturated fatty acids and inﬂammatory mediator production. The American Journal of Clinical Nutrition, 71, 1–6. https://doi.org/10.1093/ajcn/71.1.343.
Kapoor, M., Kojima, F., Appleton, I., Kawai, S., & Crofford, L. J. (2006). Major enzymatic pathway in dermal wound healing: current understanding and future therapeutic target. Current Opinion Investigational Drugs, 7, 418-422. https://pubmed.ncbi.nlm.nih.gov/16729717/
Kim, J. E., Yun, W. B., Sung, J. E., Lee, H. A., Choi, J. Y., Choi, Y. S., Jung, Y. S., Kim, K. S., Hwang, D. Y., Kirsner, R. S., & Bogensberger, G. (2002). In wound healing: alternatives in management (Kloth, L.C. and McCulloch, J.M., ed). Philadelphia: FA Davis Company.
LaMotte, R. H., Dong, X., & Ringkamp, M. (2014). Sensory neurons and circuits mediating itch. Nature Reviews Neuroscience, 15, 19–31. https://doi.org/10.1038/nrn3641
Leung, L., & Cahill, C. M. (2010). TNF-alpha and neuropathic pain - a review. Journal of Neuroinflammation, 7, 27. https://doi.org/10.1186/1742-2094-7-27
Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25, 402-8. https://doi.org/10.1006/meth.2001.1262
Mat Jais, A. M. (2007). Pharmacognosy and pharmacology of Haruan (Channa striatus), a medicinal fish with wound healing properties. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 6, 52-60. https://www.redalyc.org/articulo.oa?id=85660303
Mat Jais, A. M., Dambisya, Y. M., & Lee, T. L. (1997). Antinociceptive activity of Channa striatus (Haruan) extracts in mice. Journal of Ethnopharmacology, 57, 125–130. https://doi.org/10.1016/s0378-8741(97)00057-3
Mat Jais, A. M., McCullock, R., & Croft, K. (1994). Fatty acid and amino acid composition in Haruan as a potential role in wound healing. General Pharmacology, 25, 947 – 950. https://doi.org/10.1016/0306-3623(94)90101-5.
McMahon, S. B., & Koltzenburg, M. (1992). Itching for an explanation. Trends in Neurosciences, 15, 497-501. https://doi.org/10.1016/0166-2236(92)90102-e
Medzhitov, R. (2010). Inﬂammation 2010: new adventures of an old ﬂame. Cell, 140, 771–776. http://dx.doi.org/10.1016/j.cell.2010.03.006
Miyamoto, T., Nojima, H., Shinkado, T., Nakahashi, T., & Kuraishi, Y. (2002). Itch-associated response induced by experimental dry skin in mice. The Japanese Journal of Pharmacology, 88, 285-92. https://doi.org/10.1254/jjp.88.285
Mohd Shafri, M. A., & Mat Jais, A. M. (2012). Therapeutic potential of the haruan (Channa striatus): from food to medicinal uses. Malaysian Journal of Nutrition, 18, 125-136. https://pubmed.ncbi.nlm.nih.gov/23713236/
Mohsin, A. K., & Ambak, M. A. (1983). Freshwater fish of peninsular Malaysia (Striatus bloch). Philippines Journal of Fishery, 7, 105–140.
Nutten, S. (2015). Atopic dermatitis: global epidemiology and risk factors. Annals of Nutrition and Metabolism, 66, 8-16. https://doi.org/10.1159/000370220
Polgreen, L. E., Vehe, R. K., Rudser, K., Kunin-Batson, A., Utz, J. J., Dickson, P., Shapiro, E., & Whitley, C. B. (2016). Elevated TNF-α is associated with pain and physical disability in mucopolysaccharidosis types I, II, and VI. Molecular Genetics and Metabolism, 117, 427–430. https://doi.org/10.1016/j.ymgme.2016.01.012
Ringbom, T., Huss, U., Stenholm, A., Flock, S., Skattebøl, L., Perera, P., & Bohlin, L. (2001). Cox-2 inhibitory eﬀects of naturally occurring and modiﬁed fatty acids. Journal of Natural Products, 64, 745–749. https://doi.org/10.1021/np000620d
Wu, G. (2013). Amino acids: biochemistry and nutrition. Boca Raton: CRC Press. Taylor & Francis Group. https://doi.org/10.1201/b14661.
Yosipovitch, G., Greaves, M. W., & Schmelz, M. (2003). Itch. Lancet, 361, 690–4. https://doi.org/10.1016/S0140-6736(03)12570-6
Zakaria, Z. A., Kumar, G. H., Mat Jais, A. M., Sulaiman, M. R., & Somchit, M. N. (2008). Aniti-nociceptive, anti-inflammatory and antipyretic properties of Channa striatus filet aqueous and lipid- based extracts in rats. Methods and Findings in Experimental and Clinical Pharmacology, 30, 355-362. https://doi.org/10.1358/mf.2008.30.5.1186084
Zakaria, Z. A., Somchit, M. N., Sulaiman, M. R., & Mat Jais, A. M. (2004). Preliminary investigation on the antinociceptive properties of Haruan (Channa striatus) fillet extracted with various solvent systems. Pakistan Journal of Biological Science, 7, 1706–1710. https://dx.doi.org/10.3923/pjbs.2004.1706.1710
Zakaria, Z. A., Sulaiman, M. R., Somchit, M. N., Mat Jais, A. M., & Daud, I. A. (2005). The effects of L-arginine, D-arginine, L-NAME and methylene blue on Channa striatus–induced periph-eral antinociception in mice. Journal of Pharmacy and Pharmaceutical Sciences, 8, 199 – 206. https://pubmed.ncbi.nlm.nih.gov/16124931/
Zakaria, Z., Mat Jais, A. M., Goh, Y. M., Sulaiman, M. R. & Somchit, M. N. (2007). Amino acid and fatty acid composition of an aqueous extract of Channa striatus (Haruan) that exhibits antinociceptive activity. Clinical and Experimental Pharmacology and Physiology, 34, 198–204. http://doi.org/10.1111/j.1440-1681.2007.04572
Zuraini, A., Somchit, M. N., Solihah, M. H., Goh, Y. M., Arifah, A. K., Zakaria, M. S., Rajion, M. A., Zakaria, Z. A., & Mat Jais, A. M. (2006). Fatty acid and amino acid composition of three local Malaysian Channa spp. Fish. Food Chemistry, 97, 674 – 678. http://doi.org/10.1016/j.foodchem.2005.04.031
How to Cite
Copyright (c) 2022 Irfan Fauzi, Ahmad Akira Omar Farouk, Enoch Kumar Perimal
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The observations and associated materials published or posted by NeurosciRN are licensed by the authors for use and distribution in accord with the Creative Commons Attribution license CC BY-NC 4.0 international, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.