Neuroscience Research Notes https://neuroscirn.org/ojs/index.php/nrnotes A high quality, free open access and peer-reviewed journal from scientists to scientists. Neurotak Publishing (BC0008786-U) en-US Neuroscience Research Notes 2576-828X <p>The observations and associated materials published or posted by NeurosciRN are licensed by the authors for use and distribution in accord with the <a href="https://creativecommons.org/licenses/by-nc/4.0/" target="_blank" rel="external noopener">Creative Commons Attribution license CC BY-NC 4.0 international</a>, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</p> Free open access to liberate and unleash neglected science https://neuroscirn.org/ojs/index.php/nrnotes/article/view/4 <p>Open access has become a choice for scientific publication. In many countries, open access publication has been made compulsory for publicly funded studies. Since 2013, government-funded research paper in United States must be made freely available within 12 months of publication. Such policy will ensure maximum accessibility and circulation of published articles. While the scientific community is benefited at large due to free access and distribution of published materials, open access publication is never free due to the various costs involved in the production, permanent archival and digitalisation of scholarly articles.</p> King Hwa Ling Noraishah Mydin Abdul-Aziz Norshariza Nordin ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-03-04 2018-03-04 1 1 1 2 10.31117/neuroscirn.v1i1.4 Cellular function of satellite cells does not play a role in muscle weakness of adult Ts1Cje mice https://neuroscirn.org/ojs/index.php/nrnotes/article/view/6 <p>Down syndrome (DS) is a genetic condition resulting from triplication of human chromosome (HSA)21. Besides intellectual disability, DS is frequently associated with hypotonia. Satellite cells are the resident cells that provides robust and remarkable regenerative capacity to the skeletal muscles, and its population size has been reported to be disease-associated. &nbsp;However, little is known about the population size of satellite cells in DS and the association of its intrinsic cellular functionality and hypotonia seen in DS. Here, we studied the Ts1Cje mouse, a DS murine model displays the muscle weakness characteristic. Satellite cell populations were immunostained with Pax7 and myonuclei numbers in the Ts1Cje extensor digitorum longus muscle were assessed. Their cellular function was further determined via <em>in vitro</em> assay in high-serum conditioned medium. Subsequently, the <em>in vitro </em>self-renewal, proliferative, and differentiation activities of these myogenic precursor cells were assessed after 24, 48, and 72h using Pax7, MyoD, and Ki67 immunomarkers. Our results showed that the population and functionality of Ts1Cje satellite cell did not differ significantly when compared to the wildtype cells isolated from disomic littermates. In conclusion, our findings indicate that intrinsic cellular functionality of the satellite cells, do not contribute to muscle weakness in Ts1Cje mouse.</p> Chai Ling Lim Usman Bala Melody Pui-Yee Leong Johnson Stanslas Rajesh Ramasamy King-Hwa Ling Pike-See Cheah ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc/4.0 2018-05-15 2018-05-15 1 1 3 10 10.31117/neuroscirn.v1i1.6