The effect of exclusive breastfeeding on synaptogenesis and cell survival in mouse offspring

Authors

  • Siti Sarahdeaz Fazzaura Putri Faculty of Medicine, Universitas Sriwijaya, Gedung FK Unsri, Jalan Dr. M. Ali Komplek RSMH, Palembang, 30126, Indonesia
  • Irfannuddin Irfannuddin Faculty of Medicine, Universitas Sriwijaya, Gedung FK Unsri, Jalan Dr. M. Ali Komplek RSMH, Palembang, 30126, Indonesia
  • Yudianita Kesuma Faculty of Medicine, Universitas Sriwijaya, Gedung FK Unsri, Jalan Dr. M. Ali Komplek RSMH, Palembang, 30126, Indonesia
  • Krisna Murti Faculty of Medicine, Universitas Sriwijaya, Gedung FK Unsri, Jalan Dr. M. Ali Komplek RSMH, Palembang, 30126, Indonesia
  • Hardi Darmawan Faculty of Medicine, Universitas Sriwijaya, Gedung FK Unsri, Jalan Dr. M. Ali Komplek RSMH, Palembang, 30126, Indonesia
  • Noriyuki Koibuchi Department of Integrative Physiology, Gunma University Graduate School of Medicine, Maebashi, Gunma, 371-8511, Japan

DOI:

https://doi.org/10.31117/neuroscirn.v7i3.337

Keywords:

Breastfeeding, Neurogenesis, Cognitive development

Abstract

The first thousand days of life are critical for determining a child's cognitive development in humans. Breastfeeding may provide the nutrients needed for brain development. Cognitive function has been widely associated with neuronal turnover, driven by synaptogenesis and apoptosis. To explore this hypothesis, this study aimed to examine the effect of exclusive breastfeeding (EBF) on cognitive function and neuronal turnover in the dentate gyrus of the mice hippocampus. We conducted cognitive function assessments using the Morris Water Maze (MWM) test. We also examined neuronal apoptosis with TUNEL assay and synaptogenesis using PSD-95 antibodies. The newborn mice (mixed gender) aged 0 days were randomly divided into two groups. The first group received exclusive breastfeeding (EBF, n = 14), while the second group (MF, n = 14) received mixed breast milk and formula milk. At postnatal day 21 (PND21), the MWM test was performed, followed by an assessment of neuronal apoptosis and synaptogenesis. In the MWM test, the escape latency of the EBF group was shorter than the MF group. There was a significant increase in PSD-95 expression and a decrease in TUNEL expression in the EBF group (p < 0.001) than in the MF group. In conclusion, exclusive breastfeeding is associated with higher cognitive function. Exclusive breastfeeding affects neuronal turnover by increasing synaptogenesis and decreasing apoptosis in the dentate gyrus of the hippocampus. This has both scientific and clinical implications, pointing to nutritional practices early in life that could optimise the attainment of cognitive potential.

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Published

2024-08-05

How to Cite

Putri, S. S. F., Irfannuddin, I., Kesuma, Y., Murti, K., Darmawan, H. and Koibuchi, N. (2024) “The effect of exclusive breastfeeding on synaptogenesis and cell survival in mouse offspring”, Neuroscience Research Notes, 7(3), pp. 337.1–337.9. doi: 10.31117/neuroscirn.v7i3.337.

Issue

Vol. 7 No. 3 (2024): Regular Issue

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Research Notes

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Copyright (c) 2024 Siti Sarahdeaz Fazzaura Putri, Irfannuddin Irfannuddin, Yudianita Kesuma, Krisna Murti, Hardi Darmawan, Noriyuki Koibuchi

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