Simple methods of dissection protocols for the rapid isolation of rat dorsal root ganglia under the non-microscopic condition

Authors

  • Muhammad Naveed (1) Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China. (2) Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, China. (3) Department of Biomedical Sciences, University of Minnesota Medical School, Duluth, MN 55812, USA. https://orcid.org/0000-0002-2193-9266
  • Lei Han Department of Pharmacy, Jiangsu Health Vocational College, Nanjing, China.
  • Sobia Noreen Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
  • Chaoyue Zhao Department of Pharmacy, Jiangsu Health Vocational College, Nanjing, China.
  • Gang Sheng Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, China.
  • Lulu Xu Department of Pharmacy, Jiangsu Health Vocational College, Nanjing, China.
  • Xiaohui Zhou (1) Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China. (2) Department of Surgery, Nanjing Shuiximen Hospital, Nanjing, China.

DOI:

https://doi.org/10.31117/neuroscirn.v6i2.212

Keywords:

rat, in vivo, Dorsal root ganglion, Sensory neuron, Peripheral neuropathy, Primary culture

Abstract

The dorsal root ganglia (DRG) neurons have long been studied to advance the understanding of sensory nerve function and growth. DRG neurons are also involved in determining the fundamental mechanisms of neurodegenerative and painful disorders of the peripheral nervous system. To explore the simple methods of rapid and accurate removal of DRG in anesthetized rats with the naked eye without destroying the spinal column or blood circulation. Male Wistar rats were fixed on the operating table, lumbar DRG was removed, and sufficient DRG neurons were harvested without microscopic equipment. At the same time, the procedure was accomplished without disruption to breathing and circulation. These approaches reduce the time required for DRG collection, thereby improving efficiency, and increasing the chances of generating healthy primary DRG cultures and reproducible experiments using DRG tissue.

Author Biography

Muhammad Naveed, (1) Department of Clinical Pharmacy, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China. (2) Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, Nanjing, China. (3) Department of Biomedical Sciences, University of Minnesota Medical School, Duluth, MN 55812, USA.

Muhammad Naveed currently works at the University of Minnesota, United States. He has been the co-author of several peer-reviewed papers and book chapters. Naveed has been awarded the "Jiangsu Government Outstanding Student Scholarship" in the year of 2020. Naveed conducted research on Brain and neurosciences and heart failure.

References

Bai, X., Yu, L., Liu, Q., Zhang, J., Li, A., Han, D., . . . Gong, H. (2006). A high-resolution anatomical rat atlas. Journal of Anatomy, 209 (5), 707-708. https://doi.org/10.1111/j.1469-7580.2006.00645.x

Harrison, C., Epton, S., Bojanic, S., Green, A. L., & FitzGerald, J. J. (2018). The efficacy and safety of dorsal root ganglion stimulation as a treatment for neuropathic pain: A literature review. Neuromodulation, 21(3), 225-233. https://doi.org/10.1111/ner.12685

Kent, A. R., Min, X., Hogan, Q. H., & Kramer, J. M. (2018). Mechanisms of dorsal root ganglion stimulation in pain suppression: A computational modeling analysis. Neuromodulation, 21(3), 234-246. https://doi.org/10.1111/ner.12754

Krames, E. S. (2015). The dorsal root ganglion in chronic pain and as a target for neuromodulation: a review. Neuromodulation, 18(1), 24-32; discussion 32. https://doi.org/10.1111/ner.12247

Liem, L. (2015). Stimulation of the dorsal root ganglion. Progress in Neurological Surgery, 29, 213-224. https://doi.org/10.1159/000434673

Liu, W., Liang, X. C., Sun, Q., Wang, P. Y., Zhao, L., Huang, W. Z., & Li, B. W. (2013). [Effects of Jinmaitong capsule on oxidative stress and cell apoptosis of dorsal root ganglion in diabetic rats]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao, 35(6), 649-654. https://doi.org/10.3881/j.issn.1000-503X.2013.06.012

Liyuan, M. A., Jiang, J., Zhang, L., Xiangyu, L. I., Zheng, J., Xin, X. U., & Wang, Q. (2018). An improved method of exploring and extracting dorsal root ganglions in rats by reversely along the nerve traveling pathway. Chinese Journal of Comparative Medicine, 6, 95-97.

Malin, S. A., Davis, B. M., & Molliver, D. C. (2007). Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity. Nature Protocols, 2(1), 152-160. https://doi.org/10.1038/nprot.2006.461

Smith, D.G. and Schenk, M.P. (2001). Dissection Guide & Atlas to the Rat (1st Edition ed.): Morton Publishing Company.

Tandrup, T. (2004). Unbiased estimates of number and size of rat dorsal root ganglion cells in studies of structure and cell survival. Journal of Neurocytology, 33(2), 173-192. https://doi.org/10.1023/b:neur.0000030693.91881.53

Wang, S., Huang, L., Lei, L., Wang, H., Fan, E., & Ying, L. I. (2010). The dissection, purification and culture of dorsal root ganglion neurons from embryonic rats. Chinese Archives of Otolaryngology-Head and Neck Surgery, 12, 656-658.

Xiong, N. X., Zhao, H. Y., & Zhang, F. C. (2006). Surgical dissection of dorsal root ganglions and sympathetic ganglions in living rats. Chinese Journal of Comparative Medicine, 6, 367-368.

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Published

2023-04-29

How to Cite

Naveed, M., Han, L., Noreen, S., Zhao, C., Sheng, G., Xu, L. and Zhou, X. (2023) “Simple methods of dissection protocols for the rapid isolation of rat dorsal root ganglia under the non-microscopic condition”, Neuroscience Research Notes, 6(2), pp. 212.1–212.6. doi: 10.31117/neuroscirn.v6i2.212.

Issue

Vol. 6 No. 2 (2023): Regular Issue

Section

Technical Notes

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Copyright (c) 2023 Muhammad Naveed, Lei Han, Sobia Noreen, Chaoyue Zhao, Gang Sheng, Lulu Xu, Xiaohui Zhou

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